Online courses directory (10358)
This course is the scientific communications portion of course 7.02, Experimental Biology and Communication. Students develop their skills as writers of scientific research, skills that also contribute to the learning of the 7.02 course materials. Through in class and out of class writing exercises, students explore the genre of the research article and its components while developing an understanding of the materials covered in the 7.02 laboratory.
Each of our cells contains nearly identical copies of our genome, which provides instructions that allow us to develop and function. This course serves as an introduction to the main laboratory and theoretical aspects of genomics and is divided into themes: genomes, genetics, functional genomics, systems biology, single cell approaches, proteomics, and applications.
Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging.
Students will be exposed to all aspects of a cutting-edge technique in modern electrophysiology, in a highly structured, team oriented environment. The research projects will probe the neural mechanisms of learning and memory through tetrode array recordings coupled with patterned microstimulation. Due to the broad nature of tasks to be completed, coupled with the team oriented approach we will be employing, we are interested in students with a wide variety of laboratory experience and skill levels.
In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production.
Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized.
WARNING NOTICE
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
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